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The first attempt at modifying human DNA was performed in by Martin Cline, but the first successful and approved nuclear gene transfer in humans was performed in May The first therapeutic use of gene transfer as well as the first direct insertion of human DNA into the nuclear genome was performed by French Anderson in a trial starting in September Between and Februaryover 2, clinical trials had been conducted, more than half of them in phase I.

It should be noted that not all medical procedures that introduce alterations to a patient's genetic makeup can be considered gene therapy. Bone marrow transplantation and organ transplants in general have been found to introduce foreign DNA into patients.

Gene therapy is defined by the precision of the procedure and the intention of direct therapeutic effects. DNA must be administered, reach the damaged cells, enter the cell and either express or disrupt a protein. Multiple delivery techniques have been explored. Naked DNA approaches have also been explored, especially in the context of vaccine development. Generally, efforts focused on administering a gene that causes a needed protein to be expressed. The vector incorporates genes into chromosomes.

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