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The Earth's outer solid layer, the lithosphere, is made of rock. Rock has been used by mankind throughout history. The minerals and metals found in rocks have been essential to human civilization. The scientific study of rocks is called petrology, which is an essential component of geology.

Rocks are geologically classified according to characteristics such as mineral and chemical composition, permeability, the texture of the constituent particles, and particle size. These physical properties are the end result of the processes that formed the rocks. These events produce three general classes of rock: Granites and similar rocks, known as meta-granitoids, form much of the continental crust.

These have diverse properties, depending on their composition and the temperature and pressure conditions in which they were formed. Metamorphic rocks are formed by subjecting any rock type—sedimentary rock, igneous rock or another older metamorphic rock—to different temperature and pressure conditions than those in which the original rock was formed.

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In molecular biology, procedures and technologies are continually being developed and older technologies abandoned. For example, before the advent of DNA gel electrophoresis agarose or polyacrylamidethe size of DNA molecules was typically determined by rate sedimentation in sucrose gradients, a slow and labor-intensive technique requiring expensive instrumentation; prior to sucrose gradients, viscometry was used.

Aside from their historical interest, it is often worth knowing about older technology, as it is occasionally useful to solve another new problem for which the newer technique is inappropriate rapeutic delivery of nucleic acid polymers into a patient's cells as a drug to treat disease. The first attempt at modifying human DNA was performed in by Martin Cline, but the first successful and approved nuclear gene transfer in humans was performed in May The first therapeutic use of gene transfer as well as the first direct insertion of human DNA into the nuclear genome was performed by French Anderson in a trial starting in September Between and Februaryover 2, clinical trials had been conducted, more than half of them in phase I.

It should be noted that not all medical procedures that introduce alterations to a patient's genetic makeup can be considered gene therapy.

Bone marrow transplantation and organ transplants in general have been found to introduce foreign DNA into patients. Gene therapy is defined by the precision of the procedure and the intention of direct therapeutic effects.

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Additionally, buildings that cannot be modified to serve the functional needs of the occupants are subject to demolition. Aside from their historical interest, it is often worth knowing about older technology, as it is occasionally useful to solve another new problem for which the newer technique is inappropriate rapeutic delivery of nucleic acid polymers into a patient's cells as a drug to treat disease. Paul, Minnesota, between and mid

DNA must be administered, reach the damaged cells, enter the cell and either express or disrupt a protein. Multiple delivery techniques have been explored.

Naked DNA approaches have also been explored, especially in the context of vaccine development.

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Generally, efforts focused on administering a gene that causes a needed protein to be expressed. The vector incorporates genes into chromosomes.

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